Team:Caltech/Recipes
From 2011.igem.org
(Difference between revisions)
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<br/> | <br/> | ||
- | '''Gibson Mix(1.33x) | + | '''Gibson Mix(1.33x)''' |
* 50 μl of Taq Ligase | * 50 μl of Taq Ligase | ||
* 100 μl of 5x isothermal buffer | * 100 μl of 5x isothermal buffer | ||
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* Nuclease-free water | * Nuclease-free water | ||
* 15 μl of Master Mix Total | * 15 μl of Master Mix Total | ||
- | <br/> | + | <br/> |
+ | |||
+ | '''Phusion PCR''' | ||
+ | * 1 uL template DNA | ||
+ | * 2..5 uL fwd and rev primer | ||
+ | * 19 uL sterile water | ||
+ | * 25 uL Phusion master mix | ||
<p>'''SOC Media'''</p> | <p>'''SOC Media'''</p> |
Revision as of 18:02, 27 July 2011
Project |
Back to Timeline . Back to Methods 50% glycerol Stock:
Agar/LB plate (Autoclaved):
Ampicillin Stock
Chloramphenicol Stock
Enrichment Minimal Media
Gibson Mix(1.33x)
Phusion PCR
SOC Media for 250 ml (adapted from [http://openwetware.org/wiki/SOC OpenWetWare])
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