red light receptor
Investigators: Julia
Waiting for sequencing results of parts BBa_I15008 and BBa_I15009.
Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3.
She also did a test digest to see if the insert is in the vector.
Miniprep
Investigators: Jakob
Miniprep of cph8 from M30109 in pSB1T3
DNA concentration was measured with NanoDrop:
| samples
| DNA concentration
| 260/280
| 260/230
|
| cph8a
| 33.7
| 1.79
| 1.74
|
| cph8b
| 41.2
| 1.79
| 1.39
|
| cph8c
| 24.1
| 1.87
| 1.47
|
| cph8d
| 30
| 1.74
| 1.84
|
| cph8e
| 34
| 1.81
| 1.86
|
| cph8f
| 30
| 1.83
| 1.81
|
Testdigest
Investigators: Julia
Testdigest of cph8
- Enzyme 1: EcoRI
- Enzyme 2: PstI
Transformation
Investigators: Julia
Transformation of BBa_I15010 (in the vector pSB2K3) in cells. Sophie plated out the cells.
Comments: No colonies grew on the plates.
PCR
Investigators: Julia
New PCR for red light receptor.
Primer:
DNA template:
Comments: There were no bands on the gel.
Precipitator
Miniprep
Investigators: Sophie, Sandra
Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.
Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).
| sample / sample*
| DNA concentration
| 280/230
| 260/230
|
| M2a alpha / M2a* alpha
| 66.5 / 91.6
| 1.84 / 1.75
| 1.76 / 1.40
|
| M2a beta / M2a* beta
| 83.8 / 13.8
| 1.80 / 1.72
| 1.52 / 0.97
|
| M2a gamma / M2a* gamma
| 118.9 / 56.1
| 1.68 / 1.86
| 1.00 / 2.13
|
| M2a delta / M2a* delta
| 101.5 / 66.0
| 1.74 / 1.85
| 1.26 / 2.16
|
| M2a epsilon / M2a* epsilon
| 112.9 / 69.9
| 1.68 / 1.82
| 1.00 / 1.82
|
| M2b alpha / M2a* alpha
| 79.7 / 74.9
| 1.87 / 1.83
| 2.20 / 2.14
|
| M2b Beta/M2b* beta
| 122.3/74.4
| 1.71/1.84
| 1.04/2.18
|
| M2b gamma / M2b* gamma
| 111.2 / 72.0
| 1.70 / 1.83
| 1.08 / 1.99
|
| M2b epsilon / M2b* epsilon
| 88.5 / 90.09
| 1.72 / 1.79
| 1.19 / 1.78
|
| M3 alpha / M3* alpha
| 136.3 / 93.9
| 1.71 / 1.78
| 1.10 / 1.74
|
| M3 beta / M3* beta
| 54.7 / 79.2
| 1.91 / 1.83
| 2.03 / 2.13
|
| M3 gamma / M3* gamma
| 130.6 / 141.7
| 1.66 / 1.80
| 0.87 / 1.88
|
| M3 delta / M3* delta
| 79.6 / 82.9
| 1.76 / 1.85
| 1.27 / 2.19
|
| M3 epsilon / M3* epsilon
| 84.1 / 122.5
| 1.79 / 1.69
| 1.45 / 1.03
|
Testdigest
Investigators: Sophie
The minipreps were digested and loaded onto a gel.
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