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Team:Cornell/Week 5
From 2011.igem.org
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==Friday== | ==Friday== | ||
| - | Lab work done by: James Mathew | + | Lab work done by: James Mathew and Nancy Li |
*GFP PCR- ed off backbone, | *GFP PCR- ed off backbone, | ||
*Primer pair A and B were phosphorylated and annealed | *Primer pair A and B were phosphorylated and annealed | ||
Revision as of 17:14, 27 July 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
July 3rd - July 9th
Sunday
Monday
Tuesday
- Met with our adviser Matt and agreed on final design. Implemented the design into finalized cad tool.
Wednesday
- Submitted final microfluidic design.
Thursday
- Put together CSS template for wiki homepage and content pages, as well as starting to make graphics
Friday
Lab work done by: James Mathew and Nancy Li
- GFP PCR- ed off backbone,
- Primer pair A and B were phosphorylated and annealed
Saturday
Lab work done by: James Mathew & Charlie Chung
- GFP PCR product Gel Purified, Digested, PCR clean up
- pZE-12 Backbone plasmid was miniprepped, digested, Gel Purified
- Primer pair A and B were phosphorylated and annealed
- Ligation of all three components done overnight.
