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Team:Lyon-INSA-ENS/Realisation/Week4
From 2011.igem.org
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<p style = "line-height : 1.5em"> | <p style = "line-height : 1.5em"> | ||
| - | Miniprep of 6 iGEM parts. Digestion | + | Miniprep using the QuickPure kit of the previous 6 iGEM parts. <br/><br/> |
| + | |||
| + | Digestion with :<br/> | ||
| + | S + P : 18A, 2M, 5L<br/> | ||
| + | X + P : 22M, 2L, 24E<br/><br/> | ||
| + | |||
| + | Electrophoresis of the digested plasmids versus the non digested. | ||
| + | |||
| + | |||
</p> | </p> | ||
Revision as of 07:31, 27 July 2011
Week 4
From Monday the 27th of June to Friday the 1st of July 2011
Monday
Tuesday
Culture of NM522 cells for later transformations and Curli for extraction.
Wednesday
Miniprep, digestion and electrophoresis of the Curli plasmid.
Thursday
CaCl2 chemical transformation of some iGEM kit distribution DNA :
Plate 1 :
18A (constitutive promoter, Amp )
2M ( strong RBS, Amp )
5L ( weak RBS, Amp )
22M ( RBS+YFP, Amp)
Plate 2 :
24E (YFP, Amp + Kan )
2L (GFP, Amp )
The local newspaper "VIVA" interviewed us. They ask about our project, our team and about iGEM's organization.
Friday
Miniprep using the QuickPure kit of the previous 6 iGEM parts.
Digestion with :
S + P : 18A, 2M, 5L
X + P : 22M, 2L, 24E
Electrophoresis of the digested plasmids versus the non digested.
