Team:Freiburg/Notebook/22 July

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Meeting

attendants: Jakob, Julia, Manuel, Ruediger, Sandra, Sophie, Theo (later), Tobi time: 9:00 - 10:30

green light receptor

already done:

• CcaS was amplified again from the genomic DNA

To-do:

• 3-A-Assembly of CcaR into an empty vector with a medium promotor
• 3-A-Assembly of CcaS into an empty vector with a weak promotor
• 3-A-Assembly of CcaR and CcaS to get the final construct

blue light receptor

already done:

• Primer-Design for the Gibson-Assembly
• growth medium without tryptophane is already ordered

To-do:

• amplify the NOT-Gate

red light receptor

already done:

• one positive colony from the 3-A of ho1 and the terminator
• no positive colony from the 3-A of pcyA and the terminator
• the PCR to amplify cph8 didn't give a product, we asked the team from Mexico to send us the cph8
• the team from Upsala (Sweden) has succeeded in amplifying the cph8 (according to their notebook)

To-do:

• 3-A-Assembly of the ho1-term with a mediumPromotor-mediumRBS

Lysis cassette

already done:

• the quick change didn't work the first time, we should repeat this
• possible alternative to express the lysis cassette could be: temperature regulated RNA

To-do:

• repeat the quick change

Precipitator

already done:

• the GFP-PBD was cloned (colonies showed up)

To-do:

• test digest and sequencing of the GFP-PBD (first have a look if you see the GFP fluorescence)
• possible test of the PBD: grow the E. coli, lyse them, put the lysate into a plate (96 well?), wash the wells to get rid of the other proteins, measure the GFP fluorescence with a plate reader
• microscope the E. coli cells

other suff

• think about possible give-aways for the sponsoring video
• jacob will create a Youtube account and upload the sponsoring video
• meeting for the wiki takeover: wednesday 9:00 am (new time: 12:15)
• modelling should be started now, either Ni-binding and release or GFP-PBD binding and release, Ruediger will do this

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME