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Team:Grinnell/Notebook/Gels/DspB
From 2011.igem.org
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=Gels for DspB= | =Gels for DspB= | ||
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| + | <html> | ||
| + | <table class="gallery" frame='void' rules='none'> | ||
| + | <tr> | ||
| + | <td> | ||
| + | <a name='20110707_UBC_dspB_transformation_results_colonies_1_2_3' href='https://2011.igem.org/File:20110707_UBC_dspB_transformation_results_colonies_1_2_3.jpg'> | ||
| + | <img alt ='20110707_UBC_dspB_transformation_results_colonies_1_2_3' src='https://static.igem.org/mediawiki/2011/6/6d/20110707_UBC_dspB_transformation_results_colonies_1_2_3.jpg' /> | ||
| + | </a> | ||
| + | </td> | ||
| + | <td> | ||
| + | <a name='20110708_dspB%2BpromotersVSdspB_std' href='https://2011.igem.org/File:20110708_dspB%2BpromotersVSdspB_std.jpg'> | ||
| + | <img alt ='20110708_dspB%2BpromotersVSdspB_std' src='https://static.igem.org/mediawiki/2011/1/17/20110708_dspB%2BpromotersVSdspB_std.jpg' /> | ||
| + | </a> | ||
| + | </td> | ||
| + | <td> | ||
| + | <a name='20110708_dspB%2BPrsaA_Pxyl' href='https://2011.igem.org/File:20110708_dspB%2BPrsaA_Pxyl.jpg'> | ||
| + | <img alt ='20110708_dspB%2BPrsaA_Pxyl' src='https://static.igem.org/mediawiki/2011/b/bc/20110708_dspB%2BPrsaA_Pxyl.jpg' /> | ||
| + | </a> | ||
| + | </td> | ||
| + | <td> | ||
| + | <a name='20110709dspB%2Bpxyl_Colonies5-8' href='https://2011.igem.org/File:20110709dspB%2Bpxyl_Colonies5-8.jpg'> | ||
| + | <img alt ='20110709dspB%2Bpxyl_Colonies5-8' src='https://static.igem.org/mediawiki/2011/c/c2/20110709dspB%2Bpxyl_Colonies5-8.jpg' /> | ||
| + | </a> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> | ||
| + | Gel proving the successful transformation of <i>dspB</i> containing plasmid into <i>E. coli</i> Top10. Lane 1: ladder; lanes 2-4: digests (EcoRI and PstI) of miniprep DNA from overnights of transformants. There is a clear band at the appropriate size for <i>dspB</i> in all of the experimental lanes. | ||
| + | </td> | ||
| + | <td> | ||
| + | Gel showing <i>dspB</i> with various promoter inserts as contrasted to a standard <i>dspB</i> with no promoter. Lane 1: dspB (WT) + P<sub>rsaA</sub>; lane 2: with P<sub>xyl</sub>; lanes 3,4: with BBa_K081005; lane 5: standard <i>dspB</i>; lane 6: ladder. Not all transformants show successful insertion of the desired promoter, but it is clear which were successful when compared to the standard <i>dspB</i>. | ||
| + | </td> | ||
| + | <td> | ||
| + | Gel of transformants that should contain both <i>dspB</i> and a promoter insert. Lane 1: ladder; lanes 2-4: <i>dspB</i> with P<sub>rsaA</sub>; lanes 5-7: <i>dspB</i> with P<sub>xyl</sub>; lane 8: standard <i>dspB</i> with no promoter. | ||
| + | </td> | ||
| + | <td> | ||
| + | Gel of more transformants of <i>dspB</i> with P<sub>xyl</sub>. Lane 1: ladder; lanes 2-5: transformants; lane 6: standard <i>dspB</i> with no promoter. | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </html> | ||
| + | |||
| + | <!--###################################--> | ||
| + | |||
| + | ==2011.07.10-2011.07.16== | ||
Revision as of 16:51, 21 July 2011
Gels for DspB
2011.07.03-2011.07.09
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| Gel proving the successful transformation of dspB containing plasmid into E. coli Top10. Lane 1: ladder; lanes 2-4: digests (EcoRI and PstI) of miniprep DNA from overnights of transformants. There is a clear band at the appropriate size for dspB in all of the experimental lanes. | Gel showing dspB with various promoter inserts as contrasted to a standard dspB with no promoter. Lane 1: dspB (WT) + PrsaA; lane 2: with Pxyl; lanes 3,4: with BBa_K081005; lane 5: standard dspB; lane 6: ladder. Not all transformants show successful insertion of the desired promoter, but it is clear which were successful when compared to the standard dspB. | Gel of transformants that should contain both dspB and a promoter insert. Lane 1: ladder; lanes 2-4: dspB with PrsaA; lanes 5-7: dspB with Pxyl; lane 8: standard dspB with no promoter. | Gel of more transformants of dspB with Pxyl. Lane 1: ladder; lanes 2-5: transformants; lane 6: standard dspB with no promoter. |
