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Team:Lyon-INSA-ENS/Realisation/Week7
From 2011.igem.org
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| - | + | Start of 5mL liquid cultures of selected individual colonies. | |
| + | Extraction of the plasmids to test the presence of the insert. | ||
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Revision as of 08:28, 22 July 2011
Week 7
From Monday the 18th of July to Friday the 22nd of July 2011
Monday
Start of liquid cultures containing iGEM parts or synthesized parts in order to extract DNA later
Tuesday
Midiprep to extract DNA from the previous liquid cultures.
Wednesday
Digestion of several biobricks : RBS, GFP, YFP, Curli, OmpR234. Electrophoresis to control the digestions.
Thursday
Standard or 3A ligation of the previously cut biobricks.TSS transformation into NM522.
Friday
Start of 5mL liquid cultures of selected individual colonies. Extraction of the plasmids to test the presence of the insert.
