"
Team:Lyon-INSA-ENS/Realisation/Week5
From 2011.igem.org
(Difference between revisions)
| Line 49: | Line 49: | ||
<p style = "line-height : 1.5em"> | <p style = "line-height : 1.5em"> | ||
| - | Additional minipreps of the same 6 parts. Digestion | + | Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells. |
</p> | </p> | ||
<br/> <br/> | <br/> <br/> | ||
<h6 style="text-align :left"> Tuesday </h6> | <h6 style="text-align :left"> Tuesday </h6> | ||
| - | + | <p style = "line-height : 1.5em"> | |
| + | Transformation of the ligations into NM522 using a CaCl2 chemical transformation. | ||
| + | </p> | ||
<br/> <br/> | <br/> <br/> | ||
Revision as of 09:19, 21 July 2011
Week 5
From Monday the 4th of July to Friday the 8th of July 2011
Monday
Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells.
Tuesday
Transformation of the ligations into NM522 using a CaCl2 chemical transformation.
Wednesday
We shoot our "Cobalt Buster's Clip" which introduces, in a funny way, our team : students, instructors and advisors. We dedicate the all afternoon to this shooting.
