Uppsala-Sweden/14 July 2011

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2011-07-14
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Re-streak pSB1C3-ccaS_EcoRI, pGEM-amilGFP and pSB4A5. We were supposed to re-streak pSB1A3-amilCP and pSB1A3-PcpcG2 as well, but the transformants had no colonies at all. Suspect wrong plasmid backbone or no ligase added during the cloning attempt. To investigate this we did a transformation of the plasmid backbone (PSB1A3) and plated it on plates containing antibiotics ampicillin, chloramphenicol and kanamycin. We did plasmid prep of pSB1AK3-B1001, pSB1A2-R0011, pSB3T5-backbone, pSB4K5 backbone. New agar plates with antibiotics ampicillin, chloramphenicol and kanamycin, just made in the morning. Assembly of chromophores, step Cloning of tetR inverter into new backbone. Cloning of pSB1A3-amilCP and pSB1A3-PcpcG2 again. Overnight culture of pSB1A3 from the strain collection to test whether it’s right.
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The sequencing result arrived today around lunch time! RBS-pcyA strains we sent for sequencing were good. ccaR clone number 2 is better than clone number 1. Clone 1 of RBS-ho1 will be used in further assembly steps.

Latest revision as of 20:27, 20 July 2011







Notebook

June
MTWTFSS
    [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_June_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_June_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_June_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_June_2011&action=edit 5]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_June_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_June_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_June_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_June_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_June_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_June_2011&action=edit 12]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_June_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_June_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_June_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_June_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_June_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_June_2011&action=edit 19]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_June_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_June_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_June_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_June_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_June_2011&action=edit 26]
[http://2011.igem.org/Uppsala-Sweden/27_June_2011 27] [http://2011.igem.org/Uppsala-Sweden/28_June_2011 28] [http://2011.igem.org/Uppsala-Sweden/29_June_2011 29] [http://2011.igem.org/Uppsala-Sweden/30_June_2011 30]
July
MTWTFSS
        [http://2011.igem.org/Uppsala-Sweden/1_July_2011 1] [http://2011.igem.org/Uppsala-Sweden/2_July_2011 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_July_2011&action=edit 3]
[http://2011.igem.org/Uppsala-Sweden/4_July_2011 4] [http://2011.igem.org/Uppsala-Sweden/5_July_2011 5] [http://2011.igem.org/Uppsala-Sweden/6_July_2011 6] [http://2011.igem.org/Uppsala-Sweden/7_July_2011 7] [http://2011.igem.org/Uppsala-Sweden/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_July_2011&action=edit 10]
[http://2011.igem.org/Uppsala-Sweden/11_July_2011 11] [http://2011.igem.org/Uppsala-Sweden/12_July_2011 12] [http://2011.igem.org/Uppsala-Sweden/13_July_2011 13] [http://2011.igem.org/Uppsala-Sweden/14_July_2011 14] [http://2011.igem.org/Uppsala-Sweden/15_July_2011 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_July_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_July_2011&action=edit 17]
[http://2011.igem.org/Uppsala-Sweden/18_July_2011 18] [http://2011.igem.org/Uppsala-Sweden/19_July_2011 19] [http://2011.igem.org/Uppsala-Sweden/20_July_2011 20] [http://2011.igem.org/Uppsala-Sweden/21_July_2011 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_July_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_July_2011&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_July_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_July_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_July_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_July_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_August_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_August_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_August_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_August_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_August_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_August_2011&action=edit 21]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_August_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_August_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_August_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_August_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_August_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_August_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_August_2011&action=edit 28]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_August_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_August_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_August_2011&action=edit 31]
September
MTWTFSS
      [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_September_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_September_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_September_2011&action=edit 4]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_September_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_September_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_September_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_September_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_September_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_September_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_September_2011&action=edit 11]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_September_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_September_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_September_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_September_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_September_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_September_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_September_2011&action=edit 18]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_September_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_September_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_September_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_September_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_September_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_September_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_September_2011&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_September_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_September_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_September_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_September_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_October_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_October_2011&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_October_2011&action=edit 31]


2011-07-14

Re-streak pSB1C3-ccaS_EcoRI, pGEM-amilGFP and pSB4A5. We were supposed to re-streak pSB1A3-amilCP and pSB1A3-PcpcG2 as well, but the transformants had no colonies at all. Suspect wrong plasmid backbone or no ligase added during the cloning attempt. To investigate this we did a transformation of the plasmid backbone (PSB1A3) and plated it on plates containing antibiotics ampicillin, chloramphenicol and kanamycin. We did plasmid prep of pSB1AK3-B1001, pSB1A2-R0011, pSB3T5-backbone, pSB4K5 backbone. New agar plates with antibiotics ampicillin, chloramphenicol and kanamycin, just made in the morning. Assembly of chromophores, step Cloning of tetR inverter into new backbone. Cloning of pSB1A3-amilCP and pSB1A3-PcpcG2 again. Overnight culture of pSB1A3 from the strain collection to test whether it’s right.

The sequencing result arrived today around lunch time! RBS-pcyA strains we sent for sequencing were good. ccaR clone number 2 is better than clone number 1. Clone 1 of RBS-ho1 will be used in further assembly steps.
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