Uppsala-Sweden/5 July 2011

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2011-07-05
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The day started of by running a PCR on the DNA template of the green light sensor which includes the two genes ccaR and ccaS as well as the promotor PcpcG2 (see protocol ccaR BioBrick, protocol ccaS BioBrick, protocol PcpcG2 BioBrick).
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After lunch we did a plasmid preparation of the overnight cultures from 4/7 (purification protocol). At the end of the day we did a PCR to verify the red light sensor (cph8). We also did site specific mutagensis using PCR for the pigments amilGFP and amilCP in order to remove illegal EcoRI sites within the genes (protocol cph8, protocol amilGFP_EcoRI, protocol amilCP_EcoRI)

Latest revision as of 20:24, 20 July 2011







Notebook

June
MTWTFSS
    [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_June_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_June_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_June_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_June_2011&action=edit 5]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_June_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_June_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_June_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_June_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_June_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_June_2011&action=edit 12]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_June_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_June_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_June_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_June_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_June_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_June_2011&action=edit 19]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_June_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_June_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_June_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_June_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_June_2011&action=edit 26]
[http://2011.igem.org/Uppsala-Sweden/27_June_2011 27] [http://2011.igem.org/Uppsala-Sweden/28_June_2011 28] [http://2011.igem.org/Uppsala-Sweden/29_June_2011 29] [http://2011.igem.org/Uppsala-Sweden/30_June_2011 30]
July
MTWTFSS
        [http://2011.igem.org/Uppsala-Sweden/1_July_2011 1] [http://2011.igem.org/Uppsala-Sweden/2_July_2011 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_July_2011&action=edit 3]
[http://2011.igem.org/Uppsala-Sweden/4_July_2011 4] [http://2011.igem.org/Uppsala-Sweden/5_July_2011 5] [http://2011.igem.org/Uppsala-Sweden/6_July_2011 6] [http://2011.igem.org/Uppsala-Sweden/7_July_2011 7] [http://2011.igem.org/Uppsala-Sweden/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_July_2011&action=edit 10]
[http://2011.igem.org/Uppsala-Sweden/11_July_2011 11] [http://2011.igem.org/Uppsala-Sweden/12_July_2011 12] [http://2011.igem.org/Uppsala-Sweden/13_July_2011 13] [http://2011.igem.org/Uppsala-Sweden/14_July_2011 14] [http://2011.igem.org/Uppsala-Sweden/15_July_2011 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_July_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_July_2011&action=edit 17]
[http://2011.igem.org/Uppsala-Sweden/18_July_2011 18] [http://2011.igem.org/Uppsala-Sweden/19_July_2011 19] [http://2011.igem.org/Uppsala-Sweden/20_July_2011 20] [http://2011.igem.org/Uppsala-Sweden/21_July_2011 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_July_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_July_2011&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_July_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_July_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_July_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_July_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_August_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_August_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_August_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_August_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_August_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_August_2011&action=edit 21]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_August_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_August_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_August_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_August_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_August_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_August_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_August_2011&action=edit 28]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_August_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_August_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_August_2011&action=edit 31]
September
MTWTFSS
      [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_September_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_September_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_September_2011&action=edit 4]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_September_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_September_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_September_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_September_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_September_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_September_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_September_2011&action=edit 11]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_September_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_September_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_September_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_September_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_September_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_September_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_September_2011&action=edit 18]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_September_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_September_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_September_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_September_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_September_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_September_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_September_2011&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_September_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_September_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_September_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_September_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_October_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_October_2011&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_October_2011&action=edit 31]

2011-07-05

The day started of by running a PCR on the DNA template of the green light sensor which includes the two genes ccaR and ccaS as well as the promotor PcpcG2 (see protocol ccaR BioBrick, protocol ccaS BioBrick, protocol PcpcG2 BioBrick).

After lunch we did a plasmid preparation of the overnight cultures from 4/7 (purification protocol). At the end of the day we did a PCR to verify the red light sensor (cph8). We also did site specific mutagensis using PCR for the pigments amilGFP and amilCP in order to remove illegal EcoRI sites within the genes (protocol cph8, protocol amilGFP_EcoRI, protocol amilCP_EcoRI)
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