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JULY: WEEK 3
July, 11th
Reactions were incubated at 37°C for three hours while two medium-size agarose gels were prepared according to protocols. In the afternoon gel electrophoresis was performed.
As shown, in figure all clones were positive, so we cut and purified the bands of interest. After gel extraction, cut DNA was quantified:
Then ligations were performed in a final volume of 10 μl:
Ligations were incubated ON at 16°C. July, 12thE9, E10, E11, E12, E13, E14, E15, E16, E17, E18, 19, E20, E21, E22, E23, and E8 were transformed in 100 μl of TOP10 competent cells according to protocols. Plates were incubated ON at 37°C. July, 13th
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Team:UNIPV-Pavia/Calendar/July/settimana3
From 2011.igem.org
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| + | <p> | ||
| + | Ligations were incubated ON at 16°C. | ||
| + | </p> | ||
| + | |||
| + | |||
| + | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
| + | <a name="July.2C_12th"></a><h2> <span class="mw-headline">July, 12th</span></h2> | ||
| + | <p> | ||
| + | E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, 19, E20, E21, E22, E23, and E8 were transformed in 100 μl of TOP10 competent cells according to protocols. Plates were incubated ON at 37°C. | ||
| + | </p> | ||
| + | |||
| + | |||
| + | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
| + | <a name="July.2C_13th"></a><h2> <span class="mw-headline">July, 13th</span></h2> | ||
Revision as of 23:02, 19 July 2011
