Team:UNIPV-Pavia/Calendar/July/settimana3

(Difference between revisions)

Revision as of 21:45, 19 July 2011

UNIPV TEAM 2011

Reactions were incubated at 37°C for three hours while two medium-size agarose gels were prepared according to protocols.

In the afternoon gel electrophoresis was performed.

link alle 2 img dei gel con ling wiki

As shown, in figure all clones were positive, so we cut and purified the bands of interest.
After gel extraction, cut DNA was quantified:

@@ Line 56: / Line 56: @@
        <td>18</td>
        <td>2.5</td>
-       <td>1 EcoRI</td>
+       <td>1 XbaI</td>
-       <td>1 SpeI</td>
+       <td>1 PstI</td>
        <td>2.5</td>
        <td>25</td>
@@ Line 169: / Line 169: @@
+</table>
+</center>
+<p>
+Reactions were incubated at 37°C for three hours while two medium-size agarose gels were prepared according to protocols.
+</p>
+<p>
+In the afternoon gel electrophoresis was performed.
+</p>
+</html>
+                <html> <p> link alle 2 img dei gel con ling wiki</p>
+                </html>
+<html>
+<p>
+ As shown, in figure all clones were positive, so we cut and purified the bands of interest.
+</br>
+After gel extraction, cut DNA was quantified:
+</p>