"
Team:Freiburg/Notebook/19 July
From 2011.igem.org
(Difference between revisions)
(→Theoretical Gibson-Assembly) |
(→Theoretical Gibson-Assembly) |
||
| Line 13: | Line 13: | ||
'''Investigators: Sandra, Sophie''' | '''Investigators: Sandra, Sophie''' | ||
| - | Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor<br/> | + | Primer Degin for Blue light sensor (lovTAP) + trp promotor (BBa_K322999) and tetR Gen + tetO promotor (BBa_Q04400).<br/> |
<br/> | <br/> | ||
*LOVtap-Trp_up: gaattcgcggccgcttctagtcacacaggaaagtactatgt<br/> | *LOVtap-Trp_up: gaattcgcggccgcttctagtcacacaggaaagtactatgt<br/> | ||
Revision as of 14:15, 19 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Theoretical Gibson-Assembly
Investigators: Sandra, Sophie
Primer Degin for Blue light sensor (lovTAP) + trp promotor (BBa_K322999) and tetR Gen + tetO promotor (BBa_Q04400).
- LOVtap-Trp_up: gaattcgcggccgcttctagtcacacaggaaagtactatgt
- LOVtap-Trp_dw: tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg
- TetR-TetO_up: aaagaggagaaatactagatgtccagattag
- TetR-TetO_dw^: ctgcagcggccgctactag
^we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
