Team:Freiburg/Notebook/19 July

From 2011.igem.org

(Difference between revisions)
(Theoretical Gibson-Assembly)
(Theoretical Gibson-Assembly)
Line 18: Line 18:
*LOVtap-Trp_dw: tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg<br/>
*LOVtap-Trp_dw: tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg<br/>
*TetR-TetO_up: aaagaggagaaatactagatgtccagattag<br/>
*TetR-TetO_up: aaagaggagaaatactagatgtccagattag<br/>
-
*TetR-TetO_dw*: ctgcagcggccgctactag<br/>
+
*TetR-TetO_dw^: ctgcagcggccgctactag<br/>
-
*we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.<br/>
+
^we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.<br/>
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 14:13, 19 July 2011

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Theoretical Gibson-Assembly

Investigators: Sandra, Sophie

Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor

  • LOVtap-Trp_up: gaattcgcggccgcttctagtcacacaggaaagtactatgt
  • LOVtap-Trp_dw: tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg
  • TetR-TetO_up: aaagaggagaaatactagatgtccagattag
  • TetR-TetO_dw^: ctgcagcggccgctactag

^we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME