Team:Wageningen UR/Notebook/Proj1/July
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Yesterday's liquid cultures were miniprepped. | Yesterday's liquid cultures were miniprepped. | ||
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+ | '''July 30''' | ||
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+ | Inoculated 500 mL erlenmeyers with 100 ml LB + 100 ug/ul. ''E. coli'' was grown overnight on LB + amp plates and directly used as inoculum. All cultures were done in duplo. ''E. coli'' strains used were: | ||
+ | ptet-GFP | ||
+ | R0062-GFP | ||
+ | F2621-GFP | ||
+ | RBS-GFP | ||
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+ | The OD600 was measured 2 hours after inoculation and subsequently each hour, by taking 1 mL samples from the culture. the OD was measured with | ||
+ | Fluorescence was measured in a 96-wells plate on a fluorescent plate reader (Molecular Devices, M2 (toxicology). First blank measurements were done with 96 wells plates containing 50 ul LB+amp. The output corresponding with the specific wells was saved and used for further calculations. | ||
Revision as of 10:03, 30 July 2011
Building a Synchronized Oscillatory System
July - Synchronized Oscillatory System