Team:Warsaw/Project
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This year's goal of iGEM Warsaw team is to develop 2 foundational | This year's goal of iGEM Warsaw team is to develop 2 foundational | ||
techniques for synthetic biologists: | techniques for synthetic biologists: | ||
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- | Our first goal is to make easy and efficient protocol for cell free | + | <br> |
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+ | <div class="note">Synthetic Cloning</div> | ||
+ | <div>Our first goal is to make easy and efficient protocol for cell free | ||
cloning. It skips most basic step of classical cloning approach - | cloning. It skips most basic step of classical cloning approach - | ||
plasmid propagation in bacteria or yeast. This allows cloning of | plasmid propagation in bacteria or yeast. This allows cloning of | ||
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purification, ligation and product amplification. We'll also try to | purification, ligation and product amplification. We'll also try to | ||
deal with specificity problems of phiX29 polymerase by designing RNA | deal with specificity problems of phiX29 polymerase by designing RNA | ||
- | primers optimized for BioBrick amplification. | + | primers optimized for BioBrick amplification.</div> |
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+ | <div class="note">Expression Adaptors</div> | ||
+ | <div>Additionally we are working on expression adapters that would provide standardized protein expression from each RBS from community collection. Also we are working on adapters that increase protein expression </div> | ||
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Revision as of 23:18, 31 July 2011
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Project description
This year's goal of iGEM Warsaw team is to develop 2 foundational
techniques for synthetic biologists:
Synthetic Cloning
Our first goal is to make easy and efficient protocol for cell free
cloning. It skips most basic step of classical cloning approach -
plasmid propagation in bacteria or yeast. This allows cloning of
constructs that are toxic to host cells (i.e. nucleases or lysins) and
speeds up the whole procedure at least three times. The cell free
cloning process involves rolling circle amplification of ligation
products by phi29 polymerase. Our protocol is speed-oriented so we'll
try to optimize all time consuming steps: DNA digestion, substrate
purification, ligation and product amplification. We'll also try to
deal with specificity problems of phiX29 polymerase by designing RNA
primers optimized for BioBrick amplification.
Expression Adaptors
Additionally we are working on expression adapters that would provide standardized protein expression from each RBS from community collection. Also we are working on adapters that increase protein expression