Team:UNIPV-Pavia/Calendar/July/settimana2

(Difference between revisions)

Revision as of 15:14, 16 July 2011

UNIPV TEAM 2011

Digestions of previously purified plasmids were performed for ligations:

Reaction samples were incubated at 37°C for three hours while a small-size and a medium-size agarose gel were prepared according to protocols.

In the afternoon gel electrophoresis was performed.

As shown in figure all clones were positive, so we cut and purified the bands of interest.

@@ Line 25: / Line 25: @@
         <td><b>Kind</b></td>
         <td><b>Purified Dna (&mu;l)</b></td>
-        <td><b>H<small><sub>2</sub></small>O (&mul)</b></td>
+        <td><b>H<small><sub>2</sub></small>O (&mu;l)</b></td>
         <td><b>Enzyme 1 (&mu;l)</b></td>
         <td><b>Enzyme 2 (&mu;l)</b></td>
@@ Line 142: / Line 142: @@
 <p>
-A small-size and a medium-size agarose gel were prepared according to protocols.
+Reaction samples were incubated at 37°C for three hours while a small-size and a medium-size agarose gel were prepared according to protocols.
 </p>
+<p>
+In the afternoon gel electrophoresis was performed.
+</p>
+<center>
+   <table border="1">
+    <tr>
+        <td></html>[[Image:UNIPV_04_07_SSgel.jpg|center|Small size gel]]<html></td>
+        <td></html>[[Image:UNIPV_04_07_MSgel.jpg|center|Small size gel]]<html></td>
+    </tr>
+   </table>
+</center>
+As shown in figure all clones were positive, so we cut and purified the bands of interest.
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