Team:Freiburg/Notebook/13 July

From 2011.igem.org

(Difference between revisions)
(red light receptor)
(Miniprep)
Line 12: Line 12:
Miniprep of cph8 from M30109 in pSB1T3
Miniprep of cph8 from M30109 in pSB1T3
 +
 +
{| cellpadding="10" cellspacing="0" border="1"
 +
|samples
 +
|DNA concentration
 +
|260/280
 +
|260|230
 +
|-
 +
|cph8a
 +
|33.7
 +
|1.79
 +
|1.74
 +
|-
 +
|cph8b
 +
|41.2
 +
|1.79
 +
|1.39
 +
|-
 +
|cph8c
 +
|24.1
 +
|1.87
 +
|1.47
 +
|-
 +
|cph8d
 +
|30
 +
|1.74
 +
|1.84
 +
|-
 +
|cph8e
 +
|34
 +
|1.81
 +
|1.86
 +
|-
 +
|cph8f
 +
|30
 +
|1.83
 +
|1.81
 +
|}

Revision as of 12:12, 15 July 2011

red light receptor

Investigators: Julia

Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.

Miniprep

Investigators: Jakob

Miniprep of cph8 from M30109 in pSB1T3

samples DNA concentration 260/280 230
cph8a 33.7 1.79 1.74
cph8b 41.2 1.79 1.39
cph8c 24.1 1.87 1.47
cph8d 30 1.74 1.84
cph8e 34 1.81 1.86
cph8f 30 1.83 1.81