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Team:Freiburg/Notebook/12 July
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=Sequence Analysis= | =Sequence Analysis= | ||
==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
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Picking colonies (M2a, M2b, M3), inoculating LB (Cm) and let grow overnight. Start: 17:15 | Picking colonies (M2a, M2b, M3), inoculating LB (Cm) and let grow overnight. Start: 17:15 | ||
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Revision as of 13:31, 27 July 2011
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Contents |
Sequence Analysis
Lysis cassette
Lysis cassette and heat inducible promotor
S15b: (Lysis cassette K124014) is named K124017 in the registry Part without RBS at the beginning Part without antiholin the sequece is also mutated leading to wrong amino acids if combined with RBS there would be too many bases (8) between RBS and coding sequence part is likely to be not effective
green light receptor
CcaR
S17: (CcaR with Prefix and RBS) is ok. Check RBS. S18: seems to be ok too.
red light receptor
pycA+terminator
S24: prefix and suffix not complete
cph8
S29: Sequence without part. Has to be done again.
blue light receptor
LOV1
S33: LOVtap only
LOV3
S35: is K322999. This sequence is the LOVtap with the tryptophane promoter, RBS, mRFP and two terminators.
Experiments
green light receptor
Gel
Investigators: Jakob
Comments: Miniprep from June,12 were loaded onto a gel.
Ccas (M31, M32, M30)in pSB1C3
Image of the gel of M30-MM32 and M36-M38
Comments: All the samples were negative.
red light receptor
Gel
Investigators: Jakob
Comments: Miniprep from June,12 were loaded onto a gel. If they are positive: Sequencing
ho1+terminator (M36, M37, M38) in pSB1C3
Comments: All the samples were negative. See image above.
Precipitator
Plastic binding site
Investigators: Sophie
Picking colonies (M2a, M2b, M3), inoculating LB (Cm) and let grow overnight. Start: 17:15
