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Team:Freiburg/Notebook/15 July
From 2011.igem.org
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#DpnI digestion to digest the DNA template (methylated DNA) of the PCR to have only the new synthesized DNA strand. | #DpnI digestion to digest the DNA template (methylated DNA) of the PCR to have only the new synthesized DNA strand. | ||
| - | # | + | #After digestion with DpnI, transformation of cells with the corrected part (BBa_K08995) |
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Revision as of 09:23, 15 July 2011
Contents |
Meeting
attendants: Tobi, Theo, Julia, Rüdiger, Jakob, Sandra
green light receptor
already done:
To-do:
blue light receptor
already done:
- Transformation of Lov-Tap in cells.
To-do:
- Cloning of promotor (medium) in Lov-Tap part and perform a transformation. Plating out of the cells on trypthophan-free medium.
red light receptor
already done:
To-do:
Lysis cassette
already done:
- repressor part (BBa_K098995) has no bases between RBS (B0034) and start codon (K098997), resulting in no translation.
- quickchange of the repressor part to insert 6bp between RBS and start codon
To-do:
- DpnI digestion to digest the DNA template (methylated DNA) of the PCR to have only the new synthesized DNA strand.
- After digestion with DpnI, transformation of cells with the corrected part (BBa_K08995)
Precipitator
already done:
To-do:
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
