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Wednesday, July 13
From 2011.igem.org
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Revision as of 18:03, 14 July 2011
7-13-2011
Retrieved gel from cold room and covered with 1x TAE in gel box and heated it (by running the gel with nothing in it)
Prepared Mutagenesis PCR products from last Thursday to be run on Gel
The tubes I made up came from the following:
A = Green/ Blue tubes conatained Dr. Burkett's dNTP's B = Orange tubes contain Dr. Goode's dNTP's
I prepared 8 tubes as follows:
CA = Pink tube contains 10 microliters of the Control A sample and 2 microliters of loading dye
CB = Blue tube contains 10 microliters of the Control B sample and 2 microliters of loading dye
Rxn 1 A & B = Pink A tube contains 10 microliters of the RXN 1 A sample and 2 microliters of loading dye
Blue B tube contains 10 microliters of the RXN 1 B sample and 2 microliters of loading dye
Rxn 4 A & B = Pink A tube contains 10 microliters of the RXN 4 A sample and 2 microliters of loading dye
Blue B tube contains 10 microliters of the RXN 4 B sample and 2 microliters of loading dye
Rxn 5 A & B = Pink A tube contains 10 microliters of the RXN 5 A sample and 2 microliters of loading dye
Blue B tube contains 10 microliters of the RXN 5 B sample and 2 microliters of loading dye
These 8 tubs are located in the freezer and are ready to be run on the gel. They are in a yellow rack with a pink taped labeled with todays date and iGEM, mutagenesis PCR for gel.
Dr. Goode requested that the gel lanes be filled as follows
1. Ladder 2. CA 3. CB 4. R1A 5. R1B 6. Ladder 7. R4A 8. R4B 9. R5A 10. R5B
--Mduley 18:00, 13 July 2011 (CDT)
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