Team:Freiburg/Notebook/4 July
From 2011.igem.org
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<span style="color:blue;">Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.</Span> | <span style="color:blue;">Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.</Span> | ||
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+ | ==Sequencing== | ||
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+ | '''Investigators: Theo''' |
Revision as of 09:53, 14 July 2011
23. Labday
Digestion of PCR products
Investigators: Julia
Digest of PCR product Ccas.
Ccas:
- Enzyme 1: XbaI
- Enzyme 2: PstI
in vector pSB1C3:
- Enzyme 1: SpeI
- Enzyme 2: PstI
Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.
Sequencing
Investigators: Theo