Team:Freiburg/Notebook/4 July
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Revision as of 09:49, 14 July 2011
23. Labday
Digestion of PCR products
Investigators: Julia
Digest of PCR product Ccas.
Ccas:
- Enzyme 1: XbaI
- Enzyme 2: PstI
in vector pSB1C3:
- Enzyme 1: SpeI
- Enzyme 2: PstI
Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.