Team:Freiburg/Notebook/4 July

From 2011.igem.org

(Difference between revisions)
(Created page with "=='''23. Labday'''== ---- ==Digestion of PCR products== '''Investigators: Julia''' Digest of PCR product Ccas. Ccas: *Enzyme 1: XbaI *Enzyme 2: PstI in vector pSB1C3: *Enzy...")
(Digestion of PCR products)
Line 2: Line 2:
----
----
-
==Digestion of PCR products==
+
==<span style="color:green;">Digestion of PCR products</span>==
'''Investigators: Julia'''
'''Investigators: Julia'''

Revision as of 09:49, 14 July 2011

23. Labday


Digestion of PCR products

Investigators: Julia

Digest of PCR product Ccas.

Ccas:

  • Enzyme 1: XbaI
  • Enzyme 2: PstI

in vector pSB1C3:

  • Enzyme 1: SpeI
  • Enzyme 2: PstI

Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.