Team:Caltech/Week 4
From 2011.igem.org
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Talk to Kenny about PFGE<br/> | Talk to Kenny about PFGE<br/> | ||
Work on design of test constructs for estrogen receptor</p> | Work on design of test constructs for estrogen receptor</p> | ||
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+ | ==July 10== | ||
+ | <p>Start overnight culture of pSB4A5 for use in construction of pNT003</p> | ||
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Latest revision as of 00:11, 12 July 2011
Project |
July 3Checked Gibson transformations ResultsThere was no growth on the transformed Gibson plates. Since we did a small reaction, we used all of the reaction in transforming competent cells. The Gibson assembly likely failed, as one of the negative control plates had a single colony. However, we have had trouble with our competent cells in the past and will try plating what we have left.
July 4ResultsAfter plating the remaining 450 ul of our transformed cells:
July 5Start overnight cultures of Gibson transformations ResultsThe gel of the river DNA was inconclusive, as no bands were visible (even the weight ladder). The gel will be rerun. July 6Miniprep Gibson transformations and send off for sequencing ResultsAgain, no bands were visible on the gel. We will run the samples on a smaller gel to check the size of the DNA and confirm that there is in fact DNA in the sample. July 7Miniprep new biobricks and send off for sequencing ResultsThe smaller gel shows a smear at the top of the gel for one of the samples, indicating that this sample contains DNA that is probably of the correct size for work with the fosmid kit. All of the plates of the enrichment cultures showed growth. July 8Send off pNT001 and pNT002 for sequencing again July 10Start overnight culture of pSB4A5 for use in construction of pNT003
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