Team:Freiburg/Notebook/11 July
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==Miniprep of Promotor-Ribosome-binding-site-constructs== | ==Miniprep of Promotor-Ribosome-binding-site-constructs== | ||
+ | As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain | ||
+ | The DNA-concentrations measured were (in ng/µL): | ||
+ | |||
+ | PR1a: 89,7 | ||
+ | PR1b: 98,6 | ||
+ | |||
+ | PR2a: 78,2 | ||
+ | PR2b: 52,4 | ||
+ | |||
+ | PR3a: 25,7 | ||
+ | PR3b: 37,8 | ||
+ | |||
+ | PR4a: 45,7 | ||
+ | PR4b: 15,7 | ||
+ | |||
+ | PR5a: 35,1 | ||
+ | PR5b: 55,9 | ||
+ | |||
+ | PR6a: 45,7 | ||
+ | PR6b: 38,9 |
Revision as of 14:54, 11 July 2011
Commons
Commons
Miniprep of Promotor-Ribosome-binding-site-constructs
As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain The DNA-concentrations measured were (in ng/µL):
PR1a: 89,7 PR1b: 98,6
PR2a: 78,2 PR2b: 52,4
PR3a: 25,7 PR3b: 37,8
PR4a: 45,7 PR4b: 15,7
PR5a: 35,1 PR5b: 55,9
PR6a: 45,7 PR6b: 38,9