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Team:NTNU Trondheim/Safety
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Revision as of 09:51, 11 July 2011
Safety
Lab safety:
All our lab-team members attended a mandatory general safety course when we started university. We were schooled in lab-safety, fire-safety and first aid including CPR. In addition the Institute for Biotechnology at NTNU who are lending us their labs, gave us a thorough guide through all their labs and instructions on their own safety procedures, such as autoclaving waste and disposal of hazardous chemicals when we started working on the iGEM project.
We of course follow general lab safety and wear lab-coats, disposable gloves and safety-glasses when working with recombinant DNA and bacteria. When running gels, we use GelRed instead of ethidium bromide, as it is said not to penetrate cell membranes and thus should not be able to work as a mutagen. However, to be on the safe side, we always wear nitrile gloves when working with GelRed.
Risk Assesment
The following risk assessments for the lab procedures we are using have been made by the institute’s HSE group:
| Activity | Safety Procedures | Personal risk | Env. risk | Comment | ||
|---|---|---|---|---|---|---|
| Agarose gel electrophoresis, (GelRed) | Nitrile gloves, protective eyewear (with UV filter), face shield when needed.
Gelred used for staining | GelRed: Unknown, UV: B | A | Gelred is said not to penetrate cell membranes, and thus should not act as mutagen even if it is DNA-binding. Gloves also minimize the risk for exposure. UV damages on unprotected skins/eyes if instructions not followed | ||
| Antibiotic-stock solution, make and use of | Gloves, handle powder only inside fume hood | A | B | May cause allergic reactions if instructions not followed. May causlead to multi-resistant bacteria if not disposed correctly. | ||
| Autoclave | Thermoresistant gloves
Eyeprotection lab, Instructions posted for not opening autoclave too early and for not overfilling bottles or closing their lids completely. | C | A | Rapid pressure fall due to opening the autoclave to soon my cause hot liquid burns on eye or skin. Will not happen if instructions are followed. | ||
| Bacteria class 1 and recombinant bacteria | Autoclave accessible. Inactivation of genmanipulated bacteria in contaminated material and waste. Labcoat mandatory. Lab bench surfaces resistant to water, acid, alkali, solvents, disinfective agants, decontaminating agents and easy to clean. Transport between labs only in closed containers. Good microbiological practice. | A | A | Risk include Release of GMO to environment, bacterial infections. Low because DH5 alpha are crippled. | ||
| Use of open flames – (e.g. sterilization with bunsen burners) | Bunsen burner must not be left burning | B | A | Risks include skin burns and fire. New rules on handling installed. | ||
| General lab work | Safety rules according to risk assessment | - | - | No injuries requiring more than simple first aid in these laboratories for the past 5 years, the present rutines seem sufficient |
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