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Team:Freiburg/Notebook/15 June
From 2011.igem.org
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(Created page with "=='''??.Labday'''== ==PCR for Ccas and CcaR== '''Investigators: Julia''' Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6<br/> <br/> 32,5 µl H2O<br/> 2µ...") |
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'''Investigators: Julia''' | '''Investigators: Julia''' | ||
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| + | Ccas: cyanobacteriochrome is a green light receptor that induces the expression of pycobillisome linker protein<br/> | ||
| + | Ccar: cognate response regulator<br/> | ||
Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6<br/> | Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6<br/> | ||
| - | + | ||
32,5 µl H2O<br/> | 32,5 µl H2O<br/> | ||
2µl Primer fw<br/> | 2µl Primer fw<br/> | ||
Revision as of 09:05, 11 July 2011
??.Labday
PCR for Ccas and CcaR
Investigators: Julia
Ccas: cyanobacteriochrome is a green light receptor that induces the expression of pycobillisome linker protein
Ccar: cognate response regulator
Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6
32,5 µl H2O
2µl Primer fw
2µl Primer rev
1µl DNA ( synechocystis from Hess-Lab)
1µl dNTPs
0,5 µl Phusion
