"
Team:Freiburg/Notebook/12 June
From 2011.igem.org
(Difference between revisions)
(→picking clones) |
(→picking clones) |
||
| Line 6: | Line 6: | ||
<span style="color:blue;">Comments: On plates PR4, PR6, PR8 and PR 9 cells did not grow. More competent cells were plated out again. Succesful growing on plates PR1, PR2, PR3, PR5 and PR7</span> | <span style="color:blue;">Comments: On plates PR4, PR6, PR8 and PR 9 cells did not grow. More competent cells were plated out again. Succesful growing on plates PR1, PR2, PR3, PR5 and PR7</span> | ||
| + | |||
| + | Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells. | ||
| + | Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15) | ||
Revision as of 13:11, 8 July 2011
??.Labday
picking clones
Investigators: Theo, Sophie
Comments: On plates PR4, PR6, PR8 and PR 9 cells did not grow. More competent cells were plated out again. Succesful growing on plates PR1, PR2, PR3, PR5 and PR7
Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells. Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15)
