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Team:Nevada/Notebook/Temp
From 2011.igem.org
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| - | E. Coli | + | E. Coli <br> |
| - | Comment Here | + | Comment Here <br> |
| - | Cyano | + | Cyano <br> |
| - | Comment Here | + | Comment Here <br> |
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. | Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. | ||
Revision as of 19:46, 7 July 2011
Contents |
Temp Calender
Week 1 - June 1st-5th
E. Coli
Comment Here
Cyano
Comment Here
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
* Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP * 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations. Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.
Media
