"
Team:Paris Liliane Bettencourt/Notebook/2011/07/06/
From 2011.igem.org
(Difference between revisions)
(Created page with "<p style="display:block;"><font color="red">Babak</font> </p><p style="display:block;"><u>gel electrophoresis at 0.6% of Agarose</u> </p> <ul><li>well 1: ladder 100pb </li><li>w...") |
|||
| Line 7: | Line 7: | ||
</li><li>well 4: BBa_E0040 cut by XbaI and Pst1 | </li><li>well 4: BBa_E0040 cut by XbaI and Pst1 | ||
</li><li>well 6: pHyperSpank,121pb, cut by XbaI and Pst1, didn't work | </li><li>well 6: pHyperSpank,121pb, cut by XbaI and Pst1, didn't work | ||
| + | |||
| + | <p style="display:block;"><u>gel purification</u> | ||
| + | </p> | ||
| + | <ul><li>well 1: ladder 100pb | ||
| + | </li><li>from well 2: BBa_B4 pVeg+ SpoVg cut by EcoR1 and SpeI, concentration of DNA=6,3ng/µL after refiltration | ||
| + | </li><li>from well 4: BBa_E0040 cut by XbaI and Pst1,concentration of DNA=13ng/µL | ||
| + | </li></ul> | ||
Revision as of 15:15, 7 July 2011
Babak
gel electrophoresis at 0.6% of Agarose
- well 1: ladder 100pb
- well 2: BBa_B4 pVeg+ SpoVg cut by EcoR1 and SpeI
- well 4: BBa_E0040 cut by XbaI and Pst1
- well 6: pHyperSpank,121pb, cut by XbaI and Pst1, didn't work
gel purification
- well 1: ladder 100pb
- from well 2: BBa_B4 pVeg+ SpoVg cut by EcoR1 and SpeI, concentration of DNA=6,3ng/µL after refiltration
- from well 4: BBa_E0040 cut by XbaI and Pst1,concentration of DNA=13ng/µL
