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Revision as of 14:26, 7 July 2011 (view source) Babak (Talk | contribs) (→ligation and transformation) ← Older edit		Revision as of 11:11, 8 July 2011 (view source) AxelS (Talk | contribs) (→ligation and transformation) Newer edit →
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	== '''''ligation and transformation''''' ==		== '''''ligation and transformation''''' ==
		+	<p style="display:block;"><font color="red">Axel</font>
	<p style="display:block;"><u>ligation</u>		<p style="display:block;"><u>ligation</u>
	</p>		</p>

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Revision as of 11:11, 8 July 2011

ligation and transformation

Axel <p style="display:block;">ligation

• BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
• BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL

Babak

Gel purification

• well 1: ladder 100pb
• from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL

Digestion

• BBa_K090403 cut by EcoR1 and XbaI
• BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx
• BBa_J18913,2428pb, cut by EcoR1 and XbaI
• BBa_pHyperspank,121pb, cut by Xbal and Ptsx
• B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI