Team:Amsterdam/6 July 2011

From 2011.igem.org

(Difference between revisions)
(Created page with "=Ideas for modeling= ''Brainstorm session with Douwe Molenaar and Isoude Kuijper'' ==Steady state model of promoters== *Information needed for model **Stability ***Transcription...")
(Ideas for modeling)
Line 1: Line 1:
-
=Ideas for modeling=
+
=Ideas for modelling=
''Brainstorm session with Douwe Molenaar and Isoude Kuijper''  
''Brainstorm session with Douwe Molenaar and Isoude Kuijper''  
==Steady state model of promoters==
==Steady state model of promoters==

Revision as of 12:00, 6 July 2011

Contents

Ideas for modelling

Brainstorm session with Douwe Molenaar and Isoude Kuijper

Steady state model of promoters

  • Information needed for model
    • Stability
      • Transcription
        • Measuring with qPCR
    • Translation
      • Fluorescence
    • Growth rate
  • Information needed for all promoters
    • Inducible promoter
      • IPTG promoter is also dependable on a lactose receptor/transporter
    • Cold shock promoter
  • Temperature dependent
    • The inducible promoters are also concentration dependent
      • Need to look into literature for already available information

The cold chock promoter

  • How does this promoter work?

Cryobrick

  • What concentration is needed?
    • How many targets do the chaperone proteins have?

Enzyme activity

  • Enzyme activity is also temperature dependent