Team:Uppsala-Sweden/Project/Protocol

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Revision as of 09:05, 6 July 2011

Uppsala University.






Protocol

Our protocols are provided here in the order they were implemented.

hyperlinks here

Buffers, medium and solutions

Preparing chemically competent TOP10 cells

Tranformation of TOP10

Inoculation

Plasmid extraction

3A assembly

Agarose gel electrophoresis

PCR protocols

Gibson Assembly