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Sensor: Week 7 June 28-July 1
From 2011.igem.org
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==Tuesday, June 28== | ==Tuesday, June 28== | ||
| + | ===cI Repressor Test Assembly, Day 23=== | ||
| + | The sensor group continued their assembly of their testing construct. They placed an RBS in the K3 vector with Cro, the O<sub>R</sub> in front of the mCherry reporter, terminator, cI repressor and J23113 promoter as well as in front of the 34mCherry. Finally, the sensor group attempted (again) to put the B0032 RBS in front of the cI repressor. | ||
| + | |||
| + | {|border="1" | ||
| + | !Protocol | ||
| + | |align="center" colspan="2"|'''Part Involved with Protocol''' | ||
| + | |- | ||
| + | |rowspan="4"|Restriction Digest | ||
| + | |Inserts using XbaI and PstI: | ||
| + | |34mCherry<br />K648000+K648001 | ||
| + | |- | ||
| + | |Inserts using EcoRI and SpeI: | ||
| + | |B0032<br />B0034 | ||
| + | |- | ||
| + | |Vectors using EcoRI and XbaI: | ||
| + | |C0051<br />K3+Cro | ||
| + | |- | ||
| + | |Vector using SpeI and PstI: | ||
| + | |K3+O<sub>R</sub> | ||
| + | |- | ||
| + | |Ligation | ||
| + | |colspan="2"|B0034 + K3 + Cro<br />KOr + K648000 + K648001<br />KOr + 34mcherry<br />B0032 + C0051 | ||
| + | |- | ||
| + | |Transformation/Plating | ||
| + | |colspan="2"|The above ligations were transformed into Escherichia coli cells and plated on kanamycin resistant plates. | ||
| + | |} | ||
| + | The sensor group also transformed K3+Cro and J23113+B0031+C0051 in order to make stocks. The B0034+C0051 was grown up from stock. | ||
| + | |||
[[Team:Penn_State/Notebook| Back to Notebook]] | [[Team:Penn_State/Notebook| Back to Notebook]] | ||
Revision as of 14:39, 6 July 2011
Tuesday, June 28
cI Repressor Test Assembly, Day 23
The sensor group continued their assembly of their testing construct. They placed an RBS in the K3 vector with Cro, the OR in front of the mCherry reporter, terminator, cI repressor and J23113 promoter as well as in front of the 34mCherry. Finally, the sensor group attempted (again) to put the B0032 RBS in front of the cI repressor.
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Inserts using XbaI and PstI: | 34mCherry K648000+K648001 |
| Inserts using EcoRI and SpeI: | B0032 B0034 | |
| Vectors using EcoRI and XbaI: | C0051 K3+Cro | |
| Vector using SpeI and PstI: | K3+OR | |
| Ligation | B0034 + K3 + Cro KOr + K648000 + K648001 KOr + 34mcherry B0032 + C0051 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated on kanamycin resistant plates. | |
The sensor group also transformed K3+Cro and J23113+B0031+C0051 in order to make stocks. The B0034+C0051 was grown up from stock.
