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Team:Paris Liliane Bettencourt/Notebook/2011/07/04/
From 2011.igem.org
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</li><li>from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL | </li><li>from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL | ||
</li><li>from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL | </li><li>from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL | ||
| + | </li></ul> | ||
| + | |||
| + | <p style="display:block;"><font color="red">Babak</font> | ||
| + | |||
| + | </p><p style="display:block;"><u>Minipreps</u> | ||
| + | </p> | ||
| + | <ul><li>on BBa_K090403 (vector), concentration of DNA=174ng/µL | ||
| + | </li><li>on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL | ||
| + | </li><li>on BBa_B0015, concentration of DNA=375ng/µL | ||
| + | </li><li>on BBa_E1010 (RFP) concentration of DNA=50g/µL | ||
| + | </li><li>after Et-OH reconcentration BBa_E1010: 80ng/µL | ||
| + | </li></ul> | ||
| + | |||
| + | <p style="display:block;"><u>digestion</u> | ||
| + | </p> | ||
| + | <ul><li>well 2: BBa_E1010 cut by EcoR1 and SpeI | ||
</li></ul> | </li></ul> | ||
Revision as of 13:06, 7 July 2011
Minipreps, digestion, electrophoresis and purification
Axel
Minipreps
- on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
- on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
- on BBa_B0015, concentration of DNA=408.1ng/µL
- on BBa_E1010 concentration of DNA=330.8ng/µL
- on BBa_K145001, concentration of DNA=572.8ng/µL
digestion
- BBa_K090403 cut by EcoR1 and XbaI
- BBa_K143079 cut by EcoR1 and SpeI
- BBa_B0015 cut by EcoR1 and XbaI
- BBa_E1010 cut by EcoR1 and SpeI
- BBa_K145001 cut by EcoR1 and SpeI
gel electrophoresis at 0.6% of Agarose
- well 1: ladder 1kb
- well 2: BBa_B0015 cut by EcoR1 and XbaI
- well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
- well 4: BBa_K145001 cut by EcoR1 and SpeI
- well 5: BBa_K090403 cut by EcoR1 and XbaI
- well 6: BBa_K143033 cut by EcoR1 and SpeI
gel purification
- well 1: ladder 1kb
- from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
- from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
- from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
- from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL
Babak
Minipreps
- on BBa_K090403 (vector), concentration of DNA=174ng/µL
- on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL
- on BBa_B0015, concentration of DNA=375ng/µL
- on BBa_E1010 (RFP) concentration of DNA=50g/µL
- after Et-OH reconcentration BBa_E1010: 80ng/µL
digestion
- well 2: BBa_E1010 cut by EcoR1 and SpeI
