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Team:NTNU Trondheim/Protocols
From 2011.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
Adjust pH to 7,4 with NaOH, autoclave. | Adjust pH to 7,4 with NaOH, autoclave. | ||
| + | ==SOC:== | ||
| + | *Bacto-tryptone: 20 g/L | ||
| + | *Yeast extract: 5 g/L | ||
| + | *NaCl 0,5 g/L | ||
| + | *10 mL 250 mM KCl (25mM) → 0,186 g / 10mL H20 | ||
| + | *5 mL 2M MgCl2 (10mM) → 0,952 g / 5mL | ||
Revision as of 07:50, 5 July 2011
Protocols / Recipes used in the lab
LB Broth
- Bacto-tryptone: 10 g/L
- Yeast extract: 5 g/L
- NaCl 5 g/L
Adjust pH to 7,4 with NaOH, autoclave.
LA
- Dissolve LB
- Add agar, 15 g/L
Adjust pH to 7,4 with NaOH, autoclave.
SOC:
- Bacto-tryptone: 20 g/L
- Yeast extract: 5 g/L
- NaCl 0,5 g/L
- 10 mL 250 mM KCl (25mM) → 0,186 g / 10mL H20
- 5 mL 2M MgCl2 (10mM) → 0,952 g / 5mL
