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Team:British Columbia/Notebook/4 July 2011
From 2011.igem.org
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==July 04 2011== | ==July 04 2011== | ||
| - | + | ===Week of Jun27 - Jul04=== | |
| - | + | ====IDI1, HMG2, erg20-2==== | |
*Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. | *Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. | ||
*Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | *Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | ||
*Awaiting materials from France (erg20-2). | *Awaiting materials from France (erg20-2). | ||
Revision as of 19:18, 4 July 2011
July 04 2011
Week of Jun27 - Jul04
IDI1, HMG2, erg20-2
- Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order.
- Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order.
- Awaiting materials from France (erg20-2).
