Team:British Columbia/Notebook/4 July 2011

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(Created page with "==July 04 2011== ====IDI1, HMG2, erg20-2==== *Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. *Amplified 5 ...")
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==July 04 2011==
==July 04 2011==
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====IDI1, HMG2, erg20-2====
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====Week of Jun27 - Jul04====
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=====IDI1, HMG2, erg20-2=====
*Validated competent cells by transforming with pet100 vector containing Cineole synthase.  They are in working order.
*Validated competent cells by transforming with pet100 vector containing Cineole synthase.  They are in working order.
*Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab).  NOTE: did not properly centrifuge during DNA precipitation stage.  Will validate plasmids in yeast transformations to make sure plasmids are in working order.
*Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab).  NOTE: did not properly centrifuge during DNA precipitation stage.  Will validate plasmids in yeast transformations to make sure plasmids are in working order.
*Awaiting materials from France (erg20-2).
*Awaiting materials from France (erg20-2).

Revision as of 19:17, 4 July 2011

July 04 2011

Week of Jun27 - Jul04

IDI1, HMG2, erg20-2
  • Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order.
  • Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order.
  • Awaiting materials from France (erg20-2).