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Copenhagen/4 July 2011
From 2011.igem.org
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* We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program. | * We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program. | ||
| - | Afterwards we digested our mutants with Dnp [[Team:Copenhagen/Protocol#Mutations|Mutations]] | + | Afterwards we digested our mutants with Dnp - see protocols [[Team:Copenhagen/Protocol#Mutations|Mutations]] |
| - | * Transformed ''XL1-Blue'' competent cells - | + | * Transformed ''XL1-Blue'' competent cells - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]] |
Back to [[Team:Copenhagen/Notebook|Notebook]] | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Revision as of 12:19, 4 July 2011
Monday
The rest of the team have returned, happy, well rested and eager to begin working.
Lab Work
- We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
- We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.
Afterwards we digested our mutants with Dnp - see protocols Mutations
- Transformed XL1-Blue competent cells - see protocol Transformations
Back to Notebook
