Team:Calgary/Notebook/PostRegionals

From 2011.igem.org

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<li>Furthermore we produced a composite part Bba_K640006 composed of a response element we identified form the fad-2 gene in <i>Pseudomonas fluorescens</i>, the lacZ part, oriT, and ori1600, making it potentially a completely functional circuit which could be used to measure naphthenic acid's in solution.(Further characterization is required however and is being followed up on by the team up until the competition)</li></ul>
<li>Furthermore we produced a composite part Bba_K640006 composed of a response element we identified form the fad-2 gene in <i>Pseudomonas fluorescens</i>, the lacZ part, oriT, and ori1600, making it potentially a completely functional circuit which could be used to measure naphthenic acid's in solution.(Further characterization is required however and is being followed up on by the team up until the competition)</li></ul>
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<h2>Biotinylation</h2>
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<h2>Biotinylation</h2><a href="https://2011.igem.org/Team:Calgary/Project/ProjectPseudomonas#biotin">Click here</a>
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<li>Successfully biotinylate two new naphthenic acids (cyclohexanebutyric acid and cyclohexanecarboxylic acid) identifying the ability of our biotinylation method to be used with compounds of varying hydrophobicity.</li>
<li>Successfully biotinylate two new naphthenic acids (cyclohexanebutyric acid and cyclohexanecarboxylic acid) identifying the ability of our biotinylation method to be used with compounds of varying hydrophobicity.</li>

Revision as of 02:50, 29 October 2011


Post-Regionals

The America's Regional Competition

The University of Calgary iGEM Team had great success at the Regional Jamborree held in Indianapolis, Indiana this year, winning the Best Wiki and Best Measurement special prizes, obtaining a gold medal, and advancing as a finalist to the world's Jamborree at MIT. In the few weeks between competitions, our team has been hard at work to clarify, optimize, and rework aspects of our project. Here is a list of our major accomplishments in the last three weeks:

Prototype

  • The University of Calgary team has developed a prototype system composed of a portable potentiostat capable of making readings using our lacZ system
  • Furthermore we produced a composite part Bba_K640006 composed of a response element we identified form the fad-2 gene in Pseudomonas fluorescens, the lacZ part, oriT, and ori1600, making it potentially a completely functional circuit which could be used to measure naphthenic acid's in solution.(Further characterization is required however and is being followed up on by the team up until the competition)

Biotinylation

Click here
  • Successfully biotinylate two new naphthenic acids (cyclohexanebutyric acid and cyclohexanecarboxylic acid) identifying the ability of our biotinylation method to be used with compounds of varying hydrophobicity.
  • Characterized the binding of our biotin linker to a variety of types of beads and determined a protocol by which biotinylated hydrophobic compounds can bind to Invitrogen Dynabeads.
  • Developed and ran an immunoprecipitation protocol providing evidence for the "pull-down" of proteins which bind CHPA.

LacZ Electrochemical Detector

  • Optimized the electrochemical buffer system and electroplating methods to gain an electrochemical detection system operable at high effiency in field conditions.

Conjugation

  • Characterized our ori1600/oriT conjugation system against Berkeley's oriT submitted in 2005 (Bba_J01003), quantifying the relative rate of transformation. This data suggested our oriT construct has a higher efficiency of conjugation than Bba_J01003.
  • Optimized protocol for single colonies, and demonstrated the robustness of this conjugation system.

Naphthenic Acid BioSensor Marketing Analysis

  • In order to show the feasibility of using a naphthenic acid biosensor in a real world application, our team has prepared a report identifying a market for our biosensor, a comprehensive cost analysis of our product, and a comparison of our product to traditional and other biosensor methods already available in the market.

Microalgae Parts

  • Developed a testing construct for the algae optimized luciferase part.

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