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Team:Paris Bettencourt/Experiment/T7 diff subt subt microfluidic
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<h1>Nanotube-assisted diffusion of T7 polymerase in Microfluidics</h1> | <h1>Nanotube-assisted diffusion of T7 polymerase in Microfluidics</h1> | ||
| + | <h2>Experimental Scheme<h2> | ||
| + | <p>In order to test whether our <i>Bacillus subtilis</i> T7 emitters & receivers can form nanotubes when mixed, we mix them in microfluidic system modified from Jeff Hasty's recent <a href="http://biodynamics.ucsd.edu/pubs/articles/Mondragon11.pdf">paper</a> <a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Methodologies/Microchemostat_HastyJ#references">[1]</a>. We imaged two channels: one injected with an emitter strain marked by RFP and a receiver strain; the other channel filled with only the receiver strain. The receiver strain contains the T7 autoloop, which will when activated by T7 polymerase | ||
| + | <center><a href="https://2011.igem.org/File:Paris_microchemostat_channels_and_chambers.jpg"><img height=540px align="center" src="https://static.igem.org/mediawiki/2011/f/f9/Paris_microchemostat_channels_and_chambers.jpg"></a></center></p> | ||
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| + | <div id="citation_box"> | ||
| + | <p id="references">References</p> | ||
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| + | <li><i> Entrainment of a population of synthetic genetic oscillators.</i> Mondragón-Palomino, O., Danino, T., Selimkhanov, J., Tsimring, L. & Hasty, J. Science 333, 1315-1319 (2011).</li> | ||
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| + | </ol> | ||
| + | </div> | ||
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Revision as of 02:03, 29 October 2011
Nanotube-assisted diffusion of T7 polymerase in Microfluidics
Experimental Scheme
In order to test whether our Bacillus subtilis T7 emitters & receivers can form nanotubes when mixed, we mix them in microfluidic system modified from Jeff Hasty's recent paper [1]. We imaged two channels: one injected with an emitter strain marked by RFP and a receiver strain; the other channel filled with only the receiver strain. The receiver strain contains the T7 autoloop, which will when activated by T7 polymerase
References
- Entrainment of a population of synthetic genetic oscillators. Mondragón-Palomino, O., Danino, T., Selimkhanov, J., Tsimring, L. & Hasty, J. Science 333, 1315-1319 (2011).
