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Team:Grenoble/Projet/Device
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| - | Mercury exists in water at different oxydation levels, mainly Hg and $Hg^{2+}$ | + | Mercury exists in water at different oxydation levels, mainly $Hg$ and $Hg^{2+}$ |
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<h4>Collect, then prepare the sample</h4> | <h4>Collect, then prepare the sample</h4> | ||
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| - | A preparation phase is needed before the sample can be spread on the plate. It consists of the mercury ionization since the ionic form is generally not found in nature: it is either complexed to others ions or it forms organometallic compounds. This is the reason why mercury is so poisonous: some bacteria metabolize | + | A preparation phase is needed before the sample can be spread on the plate. It consists of the mercury ionization since the ionic form is generally not found in nature: it is either complexed to others ions or it forms organometallic compounds. This is the reason why mercury is so poisonous: some bacteria metabolize $Hg^{2+}$ into methyl-mercury, an organic compound eaten by fishes, which ones are subsequently eaten by humans. |
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<h4>Read the result</h4> | <h4>Read the result</h4> | ||
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| - | + | The water pollution test is performed by spreading the prepared sample on the device and waiting for the result (about one hour, according to the modelling). As shown in the illustration below, the red coloration of a channel indicates the presence of pollutant in the sample. The position of the colored channel on the plate gives us the amount of mercury in the water. | |
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<center><img height="350px" src="https://static.igem.org/mediawiki/2011/4/47/IMG_5961.JPG" class="bordure"/></center> | <center><img height="350px" src="https://static.igem.org/mediawiki/2011/4/47/IMG_5961.JPG" class="bordure"/></center> | ||
Revision as of 22:30, 28 October 2011
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Real application of our device
In this section of the wiki, we present how we consider the design of our device and discuss realistic application. To learn more about the market expectations, we met a safety official of ARKEMA. Here are also some information about mecury toxicity.
Mercury poisoning.
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Design of the device.
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Meeting with ARKEMA.
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Mercury poisoning.
Chemical forms
Mercury is a metal, liquid at room temperature. It easily become odorless and colorless vapor at room temperature. The exposure to mercury vapor can be really harmfull since mercury can easily reach the bloodstream by the lung, causing severe damage to kidney and brain.
Mercury pollution is an issue in air, soil and water. Here we focus on water pollution. Releases of mercury in the environment are mainly caused by the chemical and gold industry.
Mercury exists in water at different oxydation levels, mainly $Hg$ and $Hg^{2+}$
References
- PhD thesis: Faïdjiba LOE-MIE, Développement de sels d'onium chélatants et fluorogéniques pour la détection des ions mercuriques en microsystèmes.
- www.epa.gov/hg/effects.htm
Design and use of Mercuro-Coli device
Design
Our objective is to design a device for the detection and quantification of heavy metal pollution in a given sample. Our final device is intended to sense mercury, but for practical reasons, we mainly work with a harmless molecule as a proof of concept: anhydrotetracyclin. One should notice that most of the elements in the design of our device and genetic network would not change whatever the chemical that we could want to quantify.
The device presented here take into account the information provided by modelling results. The device we conceived is rectangular shape, thin, light and very cheap. It is made of machined polycarbonate. Vertical channels are engraved in the plastic.
Each channel form a specific chemical environnement: they contain distincts concentration of IPTG, such that a gradient is obtained on the whole plate.
A graduation is etched below the channels to read the concentration corresponding to the channel where the red stripe appear. The graduation is not shown on the picture above. Experiments are made at the moment to know how to draw the graduation. Even if we have not all the data needed to get an experimental graduation, our modelling work gave us that answer.
Use: prepare the sample, read the result
Packaging
Collect, then prepare the sample
A preparation phase is needed before the sample can be spread on the plate. It consists of the mercury ionization since the ionic form is generally not found in nature: it is either complexed to others ions or it forms organometallic compounds. This is the reason why mercury is so poisonous: some bacteria metabolize $Hg^{2+}$ into methyl-mercury, an organic compound eaten by fishes, which ones are subsequently eaten by humans.
Read the result
The water pollution test is performed by spreading the prepared sample on the device and waiting for the result (about one hour, according to the modelling). As shown in the illustration below, the red coloration of a channel indicates the presence of pollutant in the sample. The position of the colored channel on the plate gives us the amount of mercury in the water.
Disposal of the device
The device is disposable. Once the test is performed, autoclave the device to sterilize it.
Meeting at ARKEMA and lab's visits
 iGEM 2011 Main Page Contact Us:Clic here ! |
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