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Team:Grenoble/Projet/Modelling/Parameters
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Revision as of 20:09, 28 October 2011
Modelling - Parameters
Parameters
PDF file (detailed explanation on parameters and how we deduced them) for Toggle Switch
| kplac | pLac production rate | 600 proteins/min | [1] |
| kpTet | pTet production rate | 600 proteins/min | [1] |
| kpMer | pMer production rate | 600 proteins/min | [1] |
| δLacI | LacI degradation rate | 4.6E-2 min−1 | PDF file |
| δTetR | TetR degradation rate | 4.6E-2 min−1 | PDF file |
| δMerR | MerR degradation rate | 4.6E-2 min−1 | PDF file |
| KpLac - LacI | plac - LacI dissociation constant | 5.45E-7 M | Kyoto 10 |
| KpMer - MerR | pMer - MerR dissociation constant | 1.00E-8 M | [2] |
| KpTet - TerR | pTet - TetR dissociation constant | 5.00E-8 M | [3] |
| KLacI - IPTG | LacI - IPTG dissociation constant | 2.96E-5 M | [4] |
| KTetR - aTc | aTc - TetR dissociation constant | 1.5E-8 M | [5] |
| KHg2+ - MerR | Hg2+ - MerR dissociation constant | 1E-7 M | [2] |
| nplac | plac cooperativity number | 2,3 | [4] |
| npMer | pMer cooperativity number | 2,5 | [6] |
| npTet | pTet cooperativity number | 3 | [7] |
Working with the aTc/TetR parameters, we needed to now if our system could work with mercury. In order to see if it's applicable to mercury, we realized a sensitivity to parameters study.
References
[1] Nature. 2000 Jan 20;403(6767):335-8. A synthetic oscillatory network of transcriptional regulators. Elowitz MB, Leibler S.
[2] Mol Gen Genet. 1999 Aug;262(1):154-62. Purification and characterization of MerR, the regulator of the broad-spectrum mercury resistance genes in Streptomyces lividans 1326. Rother D, Mattes R, Altenbuchner J.
[3] David Braun et al. Parameter estimation for two synthetic gene networks: A case study. IEEE 2005.
[4] Nature 403, 339-342 (20 January 2000) Construction of a genetic toggle switch in Escherichia coli. Timothy S. Gardner Charles R. Cantor & James J. Collins
[5] O. Scholz, P. Schubert, M. Kintrup and W. Hillen, Tet repressor induction without Mg2+, Biochemistry 39 (2000), pp. 10914–10920
[6] Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex. D M Ralston and T V O'Halloran
[7] Systems analysis of a quorum sensing network: design constraints imposed by the functional requirements, network topology and kinetic constants. Goryachev AB, Toh DJ, Lee T.
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