3nd, Sept
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Begin the digestion of R0079, J23116, J37034.
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4th, Sept
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Begin the digestion of K145270, R0062, P0440 and E0420.
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5th, Sept
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Begin the digestion of K081009, E0430 and I13453.
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6th, Sept
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Today, Ms. Yang shows the protocol of connection. Like digestion, the procedure of
connection is not hard. But it takes 16 hours. So we do the connection of K081009,E0430.
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7th, Sept
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We do the transformation of our new plasmid, KE.
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8th, Sept
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We pick the right colonies from the medium, and put the tubes into shaker.
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9th, Sept
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Today, we complete the plasmid extraction of KE. Unfortunately, we find that our connection fails after the agarose gel electrophoresis. Then we redo the transformation of KE.
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10th, Sept
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We pick the right colonies from the medium, and put the tubes into shaker.
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11th, Sept
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We complete the plasmid extraction of KE. Ya-da! Our connection success! We got our first
part, KE!
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13th, Sept
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We do the connection of R0040 and K081016, R0062 and P0440, R0062 and E0420.
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14th, Sept
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We do the transformation of our new plasmid-------RK, RP, RE.
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15th, Sept
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We pick the right colonies from the medium, and put the tubes into shaker.
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16th, Sept
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We complete the plasmid extraction of RK, RP, RE. The connection of RP and RE success! We
got our next two parts-------RP and RE! However, the connection of RK fails. We have to do it
again .But we believe that our project will be finished soon!
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17th, Sept
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We do the connection of B0015 and C0079, R0079 and J37034.
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18th, Sept
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We do the transformation of our new plasmid-------CB, RJ.
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19th, Sept
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We pick the right colonies from the medium, and put the tubes into shaker (including RK).
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20th, Sept
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We complete the plasmid extraction of CB, RJ, RK. Ya-da! The connection of JC and RK
success! We got our next two parts------- CB and RK. But the connection of RJ is not successful.
Thus, we decide to use a plasmid of GFP instead of J37034.
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21st, Sept
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We find a new plasmid that can replace the part JC, so we use it instead of our new part JC
and we name it ¡°K¡±. We do the transformation of ¡°K¡± and GFP. At the same time, we do the
digestion of RK, RP, I13453, RP, RE, K145270, R0079. After the digestion, we do the connection of
IKE, RPRE, KRP.
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22nd, Sept
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We pick the right colonies from the medium, and put the tubes into shaker. And we do the
transformation of our new plasmid-------IKE, RPRE, KRP.
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23nd, Sept
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We pick the right colonies from the medium, and put the tubes into shaker. We complete
the plasmid extraction of ¡°K¡± and GFP. We do the digestion of ¡°K¡± and GFP. After that, we do the
connection of ¡°K¡± and CB, R0079 and GFP.
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24th, Sept
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We complete the plasmid extraction of IKE, RPRE, KRP, we get our new parts-------IKE,RPRE
and KRP. Then, we do the digestion of IKE, RPRE, KRP. After that, we do the connection of RKIKE,
KRPRE. At last, we do the transformation of our new plasmid-------KCB,RG.
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25th, Sept
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We do the transformation of our new plasmid-------RKIKE, KRPRE. After that, we pick the
right colonies from the medium, and put the tubes into shaker (including KCB and RG).09.26.
Sprinting
We pick the right colonies from the medium, and put the tubes into shaker (including RKIKE,
KRPRE). Then, we do the plasmid extraction of KCB and RG. Fortunately, we succeed. We get our
new parts-------KCB and RG. We do the digestion of KCB, RG. After that, we do the connection of
KCB and RG.
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27th, Sept
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We complete the plasmid extraction of RKIKE, KRPRE. we get our new parts-------RKIKE,
KRPRE. We do the transformation of our new plasmid-------KCBRG.
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28th, Sept
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We pick the right colonies from the medium, and put the tubes into shaker (including KCBRG only).
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29th, Sept
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We complete the plasmid extraction of KCBRG, we get our new parts-------KCBRG. Till now,
our project has been completed. We are so excited that we have a big dinner in the evening.
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"
