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Team:Lyon-INSA-ENS/Realisation/Week18
From 2011.igem.org
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<h6 style="text-align :left"> Monday </h6> <HR> | <h6 style="text-align :left"> Monday </h6> <HR> | ||
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| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
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| + | Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good. | ||
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<h6 style="text-align :left"> Tuesday </h6> <HR> | <h6 style="text-align :left"> Tuesday </h6> <HR> | ||
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| - | + | <p style=" line-height : 1.5em"> | |
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150. | ||
| + | Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used. | ||
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| - | <h6 style="text-align :left"> | + | <h6 style="text-align :left"> Wednesday </h6> <HR> |
| - | + | <br/> | |
| - | + | <p style=" line-height : 1.5em"> | |
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150. | ||
| + | Transformations: MC4100/p85, PHL1414+piG3. | ||
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Revision as of 17:41, 27 October 2011
Week 18
From Monday the 17th of October to Sunday the 23rd of October 2011
Monday
Strain construction
Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good.
Tuesday
Strain construction
Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150.
Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used.
Wednesday
Strain construction
Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150.
Transformations: MC4100/p85, PHL1414+piG3.
Friday
