Urea-cooler Assay data

1. Characterization of rocF and Arg box

1.1 Materials

Expression plasmids used in this study are shown in Table 1.

TABLE 1. Expression plasmids used for Charcterization of rocF and Arg box

designation	pSB3K3	pSB6A1
mock	PlacIQ	Alcohol-dehydrogenase(promoter-less)
rocF	Ptrc-rocF	Alcohol-dehydrogenase(promoter-less)
rocF+Arg box	Ptrc-rocF	Arg box

Strain MG1655 was transformed with either mock, tocF or rocF + Arg. As shown in Table 1, rocF gene was introduced on pSB3K3 and Arg boxes were introduced on pSB6A1.

1.2 Methods

Methods for this study are described here

1.3 Results

Each sample was assayed in duplicate urea concentration detected in each sample is shown in Table 2. Fig. 2 shows the average of these 2 values.

TABLE 2. Urea concentrations detected in duplicated

colony No.	mock	rocF	rocF+Arg box
#1	1.9	4.9	7.3
#2	0.75	4.3	7.2
Average	1.3	4.6	7.2
B.D.	0.80	0.44	0.080

Fig.2 The average of concentration values detected in duplicate