"
Team:Grenoble/Projet/Design/quorum
From 2011.igem.org
Maxime.huet (Talk | contribs) |
Cerevisiea (Talk | contribs) |
||
| Line 9: | Line 9: | ||
<div class="body"> | <div class="body"> | ||
| - | |||
<div class="blocbackground"> | <div class="blocbackground"> | ||
| Line 22: | Line 21: | ||
<tr> | <tr> | ||
<td> | <td> | ||
| - | <a href="https://static.igem.org/mediawiki/2011/2/27/Reseau_regulation_QS_receiving.png"><img src="https://static.igem.org/mediawiki/2011/2/27/Reseau_regulation_QS_receiving.png" width=" | + | <a href="https://static.igem.org/mediawiki/2011/2/27/Reseau_regulation_QS_receiving.png"><img src="https://static.igem.org/mediawiki/2011/2/27/Reseau_regulation_QS_receiving.png" width="450px"></a> |
</td> | </td> | ||
<td> | <td> | ||
| Line 32: | Line 31: | ||
<tr> | <tr> | ||
<td> | <td> | ||
| - | <a href="https://static.igem.org/mediawiki/2011/8/8b/Reseau_regulation_QS_receiv.png"><img src="https://static.igem.org/mediawiki/2011/8/8b/Reseau_regulation_QS_receiv.png" width=" | + | <a href="https://static.igem.org/mediawiki/2011/8/8b/Reseau_regulation_QS_receiv.png"><img src="https://static.igem.org/mediawiki/2011/8/8b/Reseau_regulation_QS_receiv.png" width="450px"></a> |
</td> | </td> | ||
<td> | <td> | ||
| Line 50: | Line 49: | ||
<tr> | <tr> | ||
<td> | <td> | ||
| - | <a href="https://static.igem.org/mediawiki/2011/8/81/Receiving_secr_bacteria.svg.png"><img src="https://static.igem.org/mediawiki/2011/8/81/Receiving_secr_bacteria.svg.png" width=" | + | <a href="https://static.igem.org/mediawiki/2011/8/81/Receiving_secr_bacteria.svg.png"><img src="https://static.igem.org/mediawiki/2011/8/81/Receiving_secr_bacteria.svg.png" width="350px"></a> |
</td> | </td> | ||
<td> | <td> | ||
| Line 59: | Line 58: | ||
</tr> | </tr> | ||
</table> | </table> | ||
| - | + | <center> | |
| - | <img src="https://static.igem.org/mediawiki/2011/e/e2/QS_details.png | + | <img height="300" src="https://static.igem.org/mediawiki/2011/e/e2/QS_details.png"/> |
| - | + | </center> | |
</div> | </div> | ||
| Line 75: | Line 74: | ||
</p> | </p> | ||
| - | <img src="https://static.igem.org/mediawiki/2011/1/1b/Details_lycopene.png | + | <img height="300" src="https://static.igem.org/mediawiki/2011/1/1b/Details_lycopene.png"/> |
</div> | </div> | ||
| Line 82: | Line 81: | ||
</div> | </div> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
</html> | </html> | ||
Revision as of 15:41, 26 October 2011
The Quorum Sensing
By coupling Toggle Switch with Quorum Sensing genes, the switch in a pathway defines a specific behavior: receiving or sending bacteria.
|
If MerR pathway is activated, CinI proteins are expressed providing to the bacteria the ability to release in the medium Quorum Sensing molecules, AHL. |
|
Whereas if LacI pathway is activated, CinR proteins are expressed in the inner bacteria. Thus, these bacteria will be able to received AHL molecule. |
Put together, both bacterial behaviors allow the formation of AHL/CinR complex in the inner receiving bacteria.
|
We make use of this complex to visualize the boundary between the two different bacterial behavior areas. |
Coloration
The two behaviors encounter each other at the boundary. At this location cells emit AHL closely to receivers.
The AHL molecules enter into the receiving cells and bind receptor protein to form a complex which up-regulates the promoter pCin. Thus, the expression of the three Lycopene synthesise enzymes is allowed. These three enzymes (crtB, crtE, crtI) produce the lycopene pigment so we can observe a red stripe at the interface between receiving and senders.
