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Team:Paris Bettencourt/Experiments/Methodologies/Integration
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<h1>Integration plasmid</h1> | <h1>Integration plasmid</h1> | ||
| - | + | Since we have a lot of problems with the clonning of our parts into replivative plasmid we created, we decided to ;anaged a integrative plasmid which could give us certainly better results since contrary to the replicative plasm we cannot observe a varialibity in its expression. To do that, we created the biobriked plasmid pDCPKO from the Bacillus Genetic Stock Center under the name pBGCS6. | |
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|+ Bacillus subtilis integration vector | |+ Bacillus subtilis integration vector | ||
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Revision as of 21:24, 28 October 2011
Integration plasmid
Since we have a lot of problems with the clonning of our parts into replivative plasmid we created, we decided to ;anaged a integrative plasmid which could give us certainly better results since contrary to the replicative plasm we cannot observe a varialibity in its expression. To do that, we created the biobriked plasmid pDCPKO from the Bacillus Genetic Stock Center under the name pBGCS6.
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At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (<partinfo>K143001</partinfo>).
After, we want to clone them into the integrative plasmid (<partinfo>K090403</partinfo>).
