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Team:UC Davis/Project
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<h1>Overview</h1> | <h1>Overview</h1> | ||
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| - | We set out to develop a | + | We set out to develop a quick, easy process for the expansion of basic parts into a part families. Our method employs a simple<a href="https://2011.igem.org/Team:UC_Davis/Protocols#ER-PCR">mutagenic PCR protocol</a> that uses standard VF2 and VR primers and materials most iGEM teams already have in their labs. We chose to prototype this process by creating a part family from the LacI promoter R0010, and to mutate GFP to visually assess our ability to create functional protein mutants.<br><br> |
| - | As of | + | As of November 2011, we have a functioning part family generation prcess and seven <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">well-characterized</a> LacI promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization. |
</div> | </div> | ||
Revision as of 02:07, 23 October 2011
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Got a favorite BioBrick? Check our our process for expanding basic parts into part families.Criteria
View our judging criteria for iGEM 2011 here.
Overview
We set out to develop a quick, easy process for the expansion of basic parts into a part families. Our method employs a simplemutagenic PCR protocol that uses standard VF2 and VR primers and materials most iGEM teams already have in their labs. We chose to prototype this process by creating a part family from the LacI promoter R0010, and to mutate GFP to visually assess our ability to create functional protein mutants.
As of November 2011, we have a functioning part family generation prcess and seven well-characterized LacI promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
As of November 2011, we have a functioning part family generation prcess and seven well-characterized LacI promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
