Team:Tec-Monterrey/projectmodeling/construct3

Our third construct ompA+sacC has the same promoter as the last two (P_BAD promoter, BBa_K206000, and its repressor protein araC, BBa_I13458 ) We decided to use an already reported part, membrane protein ompA and a signal peptide of lpp (BBa_K103006). We decided to try out our extracellular invertase “sacC” (BBa_K633003) cloned out of Zymmomonas Mobilis in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for inverted sugar.

Kyono K, Yanase H, Tonomura K, Kawasaki H and Sakai T. (1995) Cloning and characterization of Zymomonas mobilis genes encoding extracellular levansucrase and invertase. Biosci Biotechnol Biochem. Vol 59 (2):289-293

Kannan R, Mukundan G, Ait-Abdelkader N, Augier-Magro V, Baratti J and Gunasekaran P. (1995) Molecular cloning and characterization of the extracellular sucrose gene (sacC) of Zymomonas mobilis” Arch. Microbiol. Vol 163(3):195-204